3′ RACE Walking along a Large cDNA Employing Tiered Suppression PCR
نویسندگان
چکیده
منابع مشابه
3' RACE LaNe: a simple and rapid fully nested PCR method to determine 3'-terminal cDNA sequence.
3′ Rapid amplification of cDNA ends (RACE) has been an invaluable tool for molecular biologists since its inception (1). This method and the subsequent method of 3′ RACE walking (2) enable the simple and effective amplification of 3′ flanking cDNA sequence in a ligation-independent manner (3). However, despite their capacity for the suppression of nonspecific PCR products (4), they remain essen...
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From: Generation of cDNA libraries: Methods and protocols (Shao-Yao Ying Ed.) Humana press, pp. 41-50.
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2003
ISSN: 0736-6205,1940-9818
DOI: 10.2144/03344st04